Acridine Orange Hcl Hydrate
Price 1 INR/ Kilograms
Acridine Orange Hcl Hydrate Specification
- Physical Form
- Powder
- Usage
- Used for staining DNA/RNA in fluorescence microscopy and cytochemistry
- CAS No
- 65-61-2
- Ph Level
- ~6.0 (1% solution)
- Product Type
- Organic Compound Dye
- Structural Formula
- [image/structural diagram not included]
- Properties
- Fluorescent dye, soluble in water and alcohol, used as a nucleic acid-selective, cationic dye.
- Purity
- 95%
- Shelf Life
- 36 months
- Poisonous
- YES
- Application
- Biological stain, fluorescence microscopy, cell cycle determination, nucleic acid staining
- Smell
- Odorless
- Grade
- Analytical grade
- Appearance
- Reddish-orange powder
- HS Code
- 3204.1990
- Molecular Formula
- C17H19ClN3·xH2O
- Density
- Gram per cubic centimeter(g/cm3)
- Solubility
- Soluble in water and alcohol
- Storage
- Keep container tightly closed, store in a cool, dry, well-ventilated place, protect from light
- Ingredients
- Acridine orange hydrochloride hydrate
- EINECS No
- 200-617-7
- Shape
- Crystalline
- Melting Point
- 251-254°C (dec.)
- Molecular Weight
- 297.81 g/mol (anhydrous)
- Hazard Class
- Class 6.1 toxic substances
- Biological Activity
- Intercalates with DNA and RNA, emits green or orange fluorescence under UV
- Stability
- Stable under recommended storage conditions
- UN Number
- UN 2811
- Sensitivity
- Light and air sensitive
- Packaging
- Sealed bottle, various pack sizes (10g, 25g, 100g)
- Signal Word (GHS)
- Danger
- First Aid Measures
- Avoid ingestion/inhalation, rinse skin/eyes upon contact, seek medical attention if necessary
Acridine Orange Hcl Hydrate Trade Information
- Minimum Order Quantity
- 25 Kilograms
- Supply Ability
- 100 Kilograms Per Week
- Delivery Time
- 1 Week
About Acridine Orange Hcl Hydrate
ACRIDINE ORANGE HCL HYDRATE
Features:
- Longer shelf life
- Precise pH value
- Accurate composition
- Non-toxic
Exceptional Fluorescent Properties
Acridine Orange Hydrochloride Hydrate selectively binds to nucleic acids, producing distinct green or orange fluorescence depending on its interaction with DNA or RNA. This property makes it invaluable for differentiating cellular structures during fluorescence microscopy and cell cycle analysis, offering high sensitivity and specificity.
Safe and Stable Formulation
Supplied as a stable, crystalline powder with a 36-month shelf life under recommended storage conditions, this dye is packaged in sealed bottles across various pack sizes (10g, 25g, 100g). Its stability under proper storage ensures consistent performance and protects the integrity of sensitive biological experiments.
Versatile Applications in Research
This fluorescent dye is chiefly utilized for nucleic acid staining in cytochemistry, biological staining, and fluorescence microscopy. Compatible with water and alcohol-based protocols, it enables detailed observation of cellular components, supporting both qualitative and quantitative research needs.
FAQs of Acridine Orange Hcl Hydrate:
Q: How should Acridine Orange Hydrochloride Hydrate be stored to maintain its stability?
A: To ensure stability, keep the container tightly closed and store it in a cool, dry, well-ventilated area away from light and air exposure. Use the product within its 36-month shelf life for optimal results.Q: What is the primary process for using Acridine Orange in fluorescence microscopy?
A: Dissolve the required amount in water or alcohol to create a staining solution, then apply it to cells or tissue samples. Upon UV illumination, DNA will fluoresce green and RNA will fluoresce orange, enabling clear visualization of nucleic acids.Q: When is it necessary to take first aid measures while handling this product?
A: Immediate first aid is needed if the dye comes into contact with skin or eyes. Rinse the affected area thoroughly with water and seek medical advice if irritation persists. Avoid ingestion and inhalation at all times as it is classified as toxic.Q: What benefits does Acridine Orange offer for nucleic acid research?
A: Its high sensitivity and selectivity for DNA and RNA, coupled with strong fluorescent properties, allow for precise detection and differentiation of nucleic acids, enhancing the reliability of cell and molecular biology investigations.Q: Where does Acridine Orange Hydrochloride Hydrate find its main research application?
A: It is primarily used in biological and cytochemical studies for nucleic acid staining, cell cycle determination, and fluorescence microscopy in laboratories and research institutions.Q: Is Acridine Orange Hydrochloride Hydrate safe to use, and what precautions should be taken?
A: While stable under recommended conditions, it is a Class 6.1 toxic substance. Use personal protective equipment, handle only in well-ventilated areas, avoid direct exposure, and store securely to minimize risk.
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