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Iodo Nitro Tetrazolium (INT)

Name: Iodo Nitro Tetrazolium (INT)
Brand: ARNISH LABORATES PRIVATE LIMITED.
Price: 1 INR
Availability: InStock

Price 1 INR/ Kilograms

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MOQ : 25 Kilograms

Iodo Nitro Tetrazolium (INT) Specification

Application
Biochemical assays, Indicator for dehydrogenase activity, Enzyme staining

Molecular Formula
C40H32N12O4

Molecular Weight
792.77 g/mol

Shelf Life
2 years if properly stored

CAS No
660-68-4

Structural Formula
[Structural Formula Image or Details not provided]

Shape
Crystalline

Density
1.6 Gram per cubic centimeter(g/cm3)

Usage
Used for detection of NAD/NADH and NADP/NADPH-dependent dehydrogenases, often in electrophoresis or in situ staining.

Appearance
Yellow to orange crystalline powder

Solubility
Slightly soluble in water, soluble in DMSO, DMF

Poisonous
YES

Storage
Store in a cool, dry place, tightly closed; protect from light.

Grade
Analytical Reagent Grade

Purity
98%

Physical Form
Powder

Properties
Stains enzymes and protein bands, acts as an indicator and redox dye.

Product Type
Laboratory Chemical

Ingredients
Iodo Nitro Tetrazolium Salt

HS Code
29349900

Smell
Odorless

Melting Point
250 C (decomposition)

EINECS No
211-559-8

Ph Level
Neutral to slightly acidic (in solution)

UN Number
Not regulated

Synonyms
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; INT

Hazard Codes
Xi (Irritant)

Stability
Stable under recommended storage conditions.

Packaging Size
Available in 100 mg, 500 mg, 1 g and custom pack sizes.

Maximum Absorption (max)
490-500 nm (formazan product)

Light Sensitivity
Sensitive to light; decompose upon prolong exposure.

Iodo Nitro Tetrazolium (INT) Trade Information

Minimum Order Quantity
25 Kilograms

Supply Ability
100 Kilograms Per Week

Delivery Time
1 Week

About Iodo Nitro Tetrazolium (INT)

We are an unparalleled name engaged in offering premium quality IODO NITRO TETRAZOLIUM (INT) . Under most favorable conditions, it is processed by our deft professionals using excellent quality chemicals at our advanced processing unit. The offered chemical is widely used in coloration of nucleic acids. Moreover, we offer this IODO NITRO TETRAZOLIUM (INT) in different packaging options as per the needs of clients at nominal rates.

Features:

Accurate pH value
High effectiveness
Non-toxic nature
Longer shelf life

Reliable Biochemical Detection

INT is widely recognized in biochemical labs for its sensitivity in detecting NAD/NADH and NADP/NADPH-dependent dehydrogenases. Its staining properties allow clear visualization of protein bands and enzymes, making it invaluable for research and diagnostics.

Usage in Enzyme Staining

This reagent is commonly used for enzyme staining during electrophoresis and in situ assays. It reacts to produce a colored formazan product, efficiently highlighting active enzyme sites, thus accelerating data interpretation in experimental procedures.

Safe Handling and Storage

Although INT is stable when stored properly, it is sensitive to light and should be kept in tightly closed containers, away from prolonged exposure. Protective equipment is recommended during handling, as the substance is classified as an irritant and is poisonous.

FAQs of Iodo Nitro Tetrazolium (INT):

Q: How is Iodo Nitro Tetrazolium (INT) typically used in biochemical assays?

A: INT is utilized mainly for detecting dehydrogenase activity in biochemical assays by producing a colored formazan product upon reduction. It serves as an indicator for enzymatic activity detection in techniques such as electrophoresis and in situ staining.

Q: What are the recommended storage conditions for Iodo Nitro Tetrazolium?

A: INT should be stored in a cool, dry place with containers tightly closed, and protected from light to prevent decomposition. Following these recommendations sustains its stability for up to two years.

Q: When does INT decompose or degrade?

A: INT is sensitive to light and may decompose upon prolonged exposure, especially under direct light or high temperatures. Always handle in low-light environments and store properly to maintain product quality.

Q: Where can Iodo Nitro Tetrazolium be applied?

A: INT is mainly applied in biochemical, clinical, and research laboratories for enzyme staining, detection of dehydrogenase activity in biological samples, and protein visualization in electrophoretic separations.

Q: What are the steps for using INT in an enzyme staining process?

A: Typically, INT is dissolved in an appropriate solvent, such as DMSO or DMF, and applied to samples post-electrophoresis or in situ. Upon enzymatic reduction, a colored formazan precipitates, highlighting active enzyme sites for further analysis.

Q: What are the benefits of using INT in research applications?

A: Using INT provides sensitive, visible staining of protein and enzyme bands. It enables rapid assessment of NAD/NADH and NADP/NADPH enzyme activity, aiding in clear experimental interpretation and data accuracy.

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