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Coomassie Brilliant Blue R 250

Name: Coomassie Brilliant Blue R 250
Brand: ARNISH LABORATES PRIVATE LIMITED.
Price: 1 INR
Availability: InStock

Price 1 INR/ Kilograms

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MOQ : 25 Kilograms

Coomassie Brilliant Blue R 250 Specification

Usage
Electrophoresis, protein quantification, gel staining

Smell
Odorless

Molecular Formula
C45H44N3O7S2

Ingredients
Coomassie Brilliant Blue R 250

Appearance
Deep blue powder

Purity
98%

Taste
Bitter (not recommended for ingestion)

EINECS No
228-336-8

Structural Formula
Refer to product literature complex aromatic structure

Properties
High staining capacity, used for protein staining in electrophoresis

Poisonous
Non-poisonous (Handle with care)

Melting Point
~210C (decomposes)

Physical Form
Powder

Shelf Life
3 years

CAS No
6104-58-1

HS Code
3204

Density
1.18 Gram per cubic centimeter(g/cm3)

Storage
Store at room temperature, tightly sealed, protected from light

Product Type
Dye/Chemical Reagent

Application
Protein, nucleic acid staining

Grade
Analytical/Laboratory Grade

Ph Level
Neutral to slightly acidic

Solubility
Soluble in methanol, ethanol, and slightly soluble in water

Shape
Granular/Powder

Molecular Weight
825.97 g/mol

Packing
Available in 10g, 25g, 100g bottles

IUPAC Name
Ethanaminium, N-[4-(4-((diethyl(phenyl)methyl)amino)phenyl)azo)phenyl] -2-sulfonato-phenyl]-N-ethyl-N-phenyl-, inner salt

Stability
Stable under recommended storage

Absorption Maximum (max)
~595 nm

Hazard Codes
Not hazardous under normal conditions

Synonyms
Coomassie Blue R-250, Acid Blue 83, C.I. 42660

Color Index
C.I. 42660

Biological Use
Non-toxic for laboratory use; not intended for clinical or diagnostic use

Coomassie Brilliant Blue R 250 Trade Information

Minimum Order Quantity
25 Kilograms

Supply Ability
100 Kilograms Per Week

Delivery Time
1 Week

About Coomassie Brilliant Blue R 250

We are an unparalleled name engaged in offering premium quality COOMASSIE BRILLIANT BLUE R 250. Under most favorable conditions, it is processed by our deft professionals using excellent quality chemicals at our advanced processing unit. The offered chemical is widely used in coloration of nucleic acids. Moreover, we offer this COOMASSIE BRILLIANT BLUE R 250 in different packaging options as per the needs of clients at nominal rates.

Features:

Accurate pH value
High effectiveness
Non-toxic nature
Longer shelf life

Exceptional Staining Performance

Coomassie Brilliant Blue R 250 is renowned for its robust staining capabilities, making it an invaluable reagent for visualizing proteins on gel matrices in electrophoresis. Its distinct absorption characteristics ensure vivid bands, facilitating reliable quantification and identification in research applications.

User Safety and Storage Recommendations

While Coomassie Brilliant Blue R 250 is stable and non-toxic under prescribed laboratory conditions, it should always be handled with standard laboratory precautions. Store tightly sealed at room temperature, away from direct light, to maintain its quality and extend shelf life up to three years.

FAQs of Coomassie Brilliant Blue R 250:

Q: How is Coomassie Brilliant Blue R 250 typically used in laboratory processes?

A: Coomassie Brilliant Blue R 250 is primarily utilized for staining proteins and nucleic acids during electrophoresis and gel staining protocols. It enables researchers to visualize and quantify the presence of proteins by binding to amino acids and producing intense blue bands.

Q: What are the benefits of using Coomassie Brilliant Blue R 250 over other protein stains?

A: This dye offers high sensitivity, excellent reproducibility, and ease of use, providing sharp and clear staining results. Its non-toxic nature under normal laboratory conditions makes it preferable for routine protein analysis in research environments.

Q: When should Coomassie Brilliant Blue R 250 be stored and how long is its shelf life?

A: It should be stored at room temperature, protected from light, and tightly sealed to prevent degradation. Under these conditions, Coomassie Brilliant Blue R 250 maintains efficacy for up to three years.

Q: Where is Coomassie Brilliant Blue R 250 commonly applied?

A: The dye is extensively used in academic, biotechnology, and pharmaceutical research labs in India and internationally, mainly for protein, peptide, and nucleic acid detection in electrophoresis gels.

Q: What is the process for staining gels with Coomassie Brilliant Blue R 250?

A: After electrophoresis, the gel is incubated in a staining solution containing Coomassie Brilliant Blue R 250, followed by destaining to remove excess dye, yielding distinct blue protein bands for analysis.

Q: Is Coomassie Brilliant Blue R 250 hazardous to handle or poisonous?

A: Under normal laboratory usage, Coomassie Brilliant Blue R 250 is considered non-toxic and non-poisonous. However, proper handling, such as avoiding ingestion or inhalation, is recommended.

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