Coomassie Brilliant Blue G 250
Price 1 INR/ Kilograms
Coomassie Brilliant Blue G 250 Specification
- Structural Formula
- Available upon request
- EINECS No
- 228-147-3
- Poisonous
- Non-poisonous
- Usage
- For staining proteins in SDS-PAGE, isoelectric focusing gels, and other biochemical assays
- Shape
- Crystalline
- Appearance
- Dark blue powder
- Density
- 1.30 Gram per cubic centimeter(g/cm3)
- Properties
- Highly sensitive stain for proteins in electrophoresis; excellent reproducibility; strong color
- Ph Level
- Neutral (7.0 for working solution)
- CAS No
- 6104-58-1
- Application
- Protein staining in electrophoresis, laboratory analysis
- Molecular Weight
- 854.02 g/mol
- Molecular Formula
- C45H47N3O7S2
- Grade
- Laboratory/Reagent Grade
- Purity
- 90%
- Shelf Life
- 36 months
- Ingredients
- Coomassie Brilliant Blue G 250
- Smell
- Odorless
- Physical Form
- Powder
- HS Code
- 320419
- Product Type
- Synthetic Dye
- Storage
- Store in a cool, dry place, tightly closed container, away from light
- Solubility
- Soluble in water and methanol
- IUPAC Name
- [4-[(4-ethyl-3-methyl-5-oxo-1-phenyl-2-pyrazolin-1-yl)methyl]-2-methyl-phenyl]-(4-ethyl-3-methyl-5-oxo-1-phenyl-2-pyrazolin-1-yl)methyl-ammonium methyl sulfate
- Transport
- Non-hazardous for air and sea transport
- Safety Information
- Handle with standard laboratory precautions
- Absorption Maximum (max)
- 595 nm
- Color Index Number
- C.I. 42655
- Main Feature
- High sensitivity and convenience for rapid detection
- Compatibility
- Compatible with most buffered solutions
- Synonyms
- Acid Blue 90; C.I. 42655
- Hazard Class
- Not classified as hazardous
- Packaging
- Bottles; typically 10g, 25g, 100g, or bulk containers
Coomassie Brilliant Blue G 250 Trade Information
- Minimum Order Quantity
- 25 Kilograms
- Supply Ability
- 100 Kilograms Per Week
- Delivery Time
- 1 Week
About Coomassie Brilliant Blue G 250
Features:
- Accurate pH value
- High effectiveness
- Non-toxic nature
- Longer shelf life
Superior Sensitivity for Protein Analysis
Coomassie Brilliant Blue G 250 delivers outstanding sensitivity for detecting proteins in electrophoresis applications. Its formulation ensures strong staining intensity, making it easier to identify and analyze protein bands, even at lower concentrations. This high-performance reagent supports reliable, accurate biochemical results.
Safe and Convenient Laboratory Usage
This dye is non-hazardous and non-poisonous, with no odor, and requires only standard laboratory safety precautions. Compatible with diverse buffer solutions, it simplifies laboratory workflow and ensures consistent outcomes. The powder form dissolves easily, facilitating straightforward preparation of working solutions for daily use.
Export Quality Packaging and Long Shelf Life
Available in bottles of 10g, 25g, 100g, or bulk containers, Coomassie Brilliant Blue G 250 meets laboratory and export requirements. With a shelf life of 36 months and stability in cool, dry conditions, it remains effective for extended periods. Packaging is designed for safety, ease of transport, and secure storage.
FAQs of Coomassie Brilliant Blue G 250:
Q: How is Coomassie Brilliant Blue G 250 used for protein staining?
A: Coomassie Brilliant Blue G 250 is dissolved in buffered solutions, typically at neutral pH, and applied to electrophoresis gels such as SDS-PAGE or isoelectric focusing gels. It selectively binds to proteins, allowing visible staining of protein bands for analysis.Q: What benefits does Coomassie Brilliant Blue G 250 offer in biochemical assays?
A: The dye provides high sensitivity, excellent color intensity, and reproducibility, enabling rapid and reliable detection of proteins, even at low concentrations. Its strong performance ensures dependability in laboratory research.Q: When should I use Coomassie Brilliant Blue G 250 in my experiments?
A: It is recommended for staining proteins following electrophoresis or biochemical separation techniques. Use it in laboratory assays where sensitive, easy-to-use protein detection is essential.Q: Where can Coomassie Brilliant Blue G 250 be stored?
A: Store Coomassie Brilliant Blue G 250 in a tightly closed container, placed in a cool, dry area away from direct light. Proper storage maintains its performance and extends its shelf life to up to 36 months.Q: What safety precautions are required when handling Coomassie Brilliant Blue G 250?
A: Follow standard laboratory safety measures, such as wearing gloves and lab coats. Coomassie Brilliant Blue G 250 is not classified as hazardous or poisonous, but routine care and good laboratory practices should still be observed.Q: Is Coomassie Brilliant Blue G 250 compatible with other reagents or buffered solutions?
A: Yes, the dye is compatible with most buffered solutions commonly used in protein staining and electrophoresis, allowing flexible integration with established lab protocols.Q: What is the process for preparing a working solution of Coomassie Brilliant Blue G 250?
A: To prepare the working solution, dissolve the powder in water or methanol according to laboratory protocol, ensuring a neutral pH (around 7.0). Use immediately or store in appropriate conditions as recommended.
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